Fig. 2: Rational optimization yields the discovery of highly potent seed germination stimulants for P. aegyptiaca and S. hermonthica.

a Structural optimization process of compounds 3f and 3g. b Competitive ShHTL7 inhibitory activity curve via YLG assay under different concentrations of (R)-4a and (S)-4a, error bars mean SD (n = 3 biologically independent experiments). c The P. aegyptiaca germination EC50 values of compounds 4a, 4b, (R)-4a, (S)-4a, and rac-GR24, error bars mean SD (n = 3 biologically independent experiments). d The S. hermonthica germination EC50 values of compounds 4a, (R)-4a, (S)-4a, and rac-GR24, error bars mean SD (n = 2 or 3 biologically independent experiments).