Fig. 1: Epithelium-specific ACC1 deletion affects crypt architecture in the small intestine and the colon.

ACC1^Δ/ΔIEC and ACC1^lox/lox control mice were treated with tamoxifen (i.p.) for 5 consecutive days and analyzed on day 9 upon the last tamoxifen injection. a RNA was extracted from duodenum, ileum and colon epithelial cells and qPCR was performed to determine ACC1 deletion efficiency. Data was pooled from 2 independent experiments with a total of n = 9 and 10 mice per group. b Representative H&E stainings of duodenum, jejunum and ileum and c of the proximal (prox.) and distal (dist.) part of colon from ACC1^lox/lox control (upper panel) or ACC1^Δ/ΔIEC mice (lower panel) from >3 independent experiments with n ≥ 3 mice per group are shown. Scale bars represent 100 µm. d Leukocytes were isolated from the lamina propria of the colon and small intestine and analyzed by flow cytometry. Representative flow cytometry plots from ACC1^lox/lox control and ACC1^Δ/ΔIEC mice are shown (upper panel). Graphs show frequency (%) of live CD45⁺ T cells (T), B cells (B), dendritic cells (DC), neutrophilic granulocytes (Gr) and macrophages (MO), Data was pooled from 2 independent experiments with a total of n = 8 and 9 mice per group. Statistical significance was analyzed using unpaired two-tailed Student’s t-test (a, d) with *p < 0.05, **p < 0.01, ***p < 0.001. ****p < 0.0001. Exact p values provided as Source Data. Bar graphs represent mean and error bars indicate SD. Source data are provided as a Source Data file.