Fig. 3: Base editing in the mitochondrial genome induced by mDdCBEs and DdCBEs.
From: Base editing in human cells with monomeric DddA-TALE fusion deaminases
a, c, e Editing efficiencies of mDdCBEs and DdCBEs at the ND4 (a), ND6 (c), and ND1 (e) sites in HEK293T cells. Target cytosines and TALE-binding sites are shown red and gray, respectively. The left and right TALE arrays are represented by L and R, respectively. Mismatches between the site recognized by the ND6-specific TALE array and the reference genome are underlined and shown in purple. b, d, f Heat maps showing the frequencies of C-to-T conversions at the indicated positions in the ND4 (b), ND6 (d), and ND1 (f) sites. The cytosine conversion rates were measured by targeted deep sequencing. Means ± s.e.m. (a, c, e) and heat map colors (b, d, f) were determined from three independent experiments. Source data are provided with this paper.
