Fig. 4: mDdCBE allows base editing in mtDNA at sites with a single TALE binding site.
From: Base editing in human cells with monomeric DddA-TALE fusion deaminases
a Among all TC motifs in human mtDNA, 8.4% can potentially be edited by mDdCBE but not DdCBE. b Schematic of a mDdCBE target site in the MT-TC gene in human mtDNA. Target cytosines are shown in red. c Editing frequencies induced by mDdCBEs at the MT-TC site in HEK293T cells. d Heat map showing the frequencies of C-to-T substitutions at various positions in the MT-TC site. The cytosine conversion rates were measured by targeted deep sequencing. Means ± s.e.m. (c) and colors in the heat map (d) were determined from three independent experiments. Source data are provided with this paper.
