Fig. 6: Mitochondrial genome-wide analysis of off-target editing by DdCBEs and mDdCBEs.
From: Base editing in human cells with monomeric DddA-TALE fusion deaminases
a, b Average frequencies of off-target C•G-to-T•A editing in mtDNA. HEK293T cells were transfected with plasmids encoding DdCBEs, mDdCBEs, TALE-free MTS-split DddAtox-UGI, and TALE-free MTS-non-split DddA–UGI targeted to the ND1 (a) and ND6 (b) sites. c Editing frequencies at the C6 and C7 positions in the ND6 site following transfection of various concentrations of mDdCBE-encoding mRNAs into HEK293T cells. d Average frequencies of off-target C•G-to-T•A editing in mtDNA from HEK293T cells transfected with mDdCBE-encoding mRNA. The cytosine conversion rates were measured by targeted deep sequencing. Means ± s.e.m. were determined from three (c) independent experiments. a, b, d Data are shown as the mean from two independent experiments. Source data are provided with this paper.
