Fig. 1: Polymorphic fibrils of α-syn formed in the presence of heparin.
From: Heparin induces α-synuclein to form new fibril polymorphs with attenuated neuropathology
a ThT kinetic assay for α-syn aggregation in the presence of a gradient concentration of heparin. α-Syn concentration is 50 μM. Data shown are mean ± SD, n = 5 independently prepared samples. b Representative negative-staining TEM images of apo-α-syn fibrils (top) and hep-α-syn fibrils (bottom) from three biologically independent experiments. Scale bar = 100 nm. c Cryo-EM micrographs of hep-α-syn and apo-α-syn fibrils. Representative 2D class averages of each polymorph are shown as insets. Scale bar = 100 nm. d Central slices of the 3D maps of each polymorph of hep-α-syn fibrils and apo-α-syn fibrils. The proportions of each polymorph in the fibril sample are indicated. Arrows indicate the additional densities.
