Fig. 2: Selection of antigens for the SONIA neutralization PCR test. | Nature Communications

Fig. 2: Selection of antigens for the SONIA neutralization PCR test.

From: Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR

Fig. 2

Both S1 subunit and receptor binding domain (RBD) of spike protein have the ability to engage with the ACE2. To evaluate suitability for the neutralization PCR assay, we tested convalescent COVID-19 patient sera (N = 2, blue circle) and healthy donor sera (N = 4, red circle) collected prior to the outbreak. The COVID-19 sera were confirmed to harbor NAb by a pseudo-virus neutralization assay. The y-axis is the SONIA neutralization PCR test signal Δ\(\overline{{Ct}}\) calculated by subtracting the Ct value of the sample from that of a buffer only blank control.

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