Fig. 6: SOX17 regulates PDGFB signaling in the NCL mesenchyme.
From: A SOX17-PDGFB signaling axis regulates aortic root development
a Representative RNAscope ISH images (n = 3/group) of E13.5 control and Sox17eKO hearts show diminished Pdgfb expression (red) in the aortic valve endothelium in Sox17eKO hearts. Noted that only the large dots are considered the real signals. b, c IF images of E15.5 control and Sox17eKO hearts show significantly reduced phosphorylated ERK (pERK) density (red) in the NCL mesenchyme of Sox17eKO hearts. (n = 4/group, mean ± SD, unpaired two-tailed t-test, p = 0.011, *p < 0.05). d, e IF images of E14.5 control and Sox17eKO hearts show significantly reduced PDGFRB density (red) in the aortic valve region, especially in NCL, of the Sox17eKO hearts. (n = 5/group, mean ± SD, unpaired two-tailed t-test, p = 0.007 for RCL, p = 0.006 for LCL, p = 0.0001 for NCL, *p < 0.05). Source data are provided as a Source Data file. Scale bars: 100 µm.
