Fig. 4: Phosphoproteomics of A. castellanii encystment.

a Normalized distribution of the protein relative quantities (iBAQ) from the entire proteome and the subset of proteins also quantified in the phosphoproteome (in black and turquoise, respectively). b Normalized distribution of the log2-transformed fold changes relative to T0 control in the phosphoproteome in the control condition (left) and during encystment (right). Time points are indicated next to the corresponding density. c Clusters of significantly regulated phospho-peptides. The dot plot is color-coded with the mean of log2-transformed fold change relative to the control condition (0 h) for each cluster during encystment and in the control condition (1, 4, 8 h). The size of each dot is proportional to the number of phosphorylation sites per cluster (indicated on the right). Significantly enriched GO terms in the different protein clusters are present on the right side of the plot (cellular component, biological process and molecular function are represented with a circle, a triangle and a square, respectively). The detailed outputs of the enrichment can be found in Supplementary Data 2 file. d Dot plot representing the phosphatase activity (in pmol phosphate/min/µg) of A. castellanii protein samples obtained one hour after encystment induction (“E”, orange) or growth medium renewal (“C”, gray). n = 6 independent experiments; median values are presented by a horizontal bar; “*”: Mann–Whitney one-sided test p-value = 0.0325. e Log2-transformed MS signal relative to the median of each replicate for the proteins discussed in the text and their regulated phosphorylation sites. Time points are indicated in the bottom: 0 h control and 1, 4, 8 h in control or encysting condition (gray and orange, respectively).