Table 3 List of chromosomal mutations identified in the genome of the SCV morphotype (n°2) when compared to the genome of morphotype n°1.

From: Personalized bacteriophage therapy to treat pandrug-resistant spinal Pseudomonas aeruginosa infection

Gene

Protein

Mutation

Effect on the protein

Impact on the protein function

Hypothetical protein

Unknown

99T > C

synonymous variant

Unlikely

sdhA

Succinate dehydrogenase flavoprotein subunit

1673A > G

missense variant: His558Arg

Unlikely: same family of aminoacids

serA

Phosphoglycerate deshydrogenase

40T > C

missense variant: Phe14Leu

Possible: different families of aminoacids

Hypothetical protein

Unknown

739A > C

missense variant: Thr247Pro

Unknown

pbuE

Purine efflux pump PbuE

605G > AT

missence variant:

insertion of Arg in 202

Unlikely (end of protein)

nodD2

Nodulation protein

303G > T

synonymous variant

Unlikely

lgrD

non-ribosomal peptide synthetase

2668A > T

stop gained Lys890*

Possible: truncated protein of 890 amino acids versus 991

  1. Variant calling was performed using the Snippy software (https://github.com/tseemann/snippy) after annotation of the reference genome of morphotype n°1 (Prokka software).