Fig. 1: Low-affinity receptors enhance IAV binding rate in presence of a low density of high-affinity receptors. | Nature Communications

Fig. 1: Low-affinity receptors enhance IAV binding rate in presence of a low density of high-affinity receptors.

From: Human-type sialic acid receptors contribute to avian influenza A virus binding and entry by hetero-multivalent interactions

Fig. 1: Low-affinity receptors enhance IAV binding rate in presence of a low density of high-affinity receptors.The alternative text for this image may have been generated using AI.

a–f, ~100 pM suspensions of human H3N2 strain VI75 (a, c, e) or avian H5N1 strain HU02 (b, d, f) were associated for 600 s to BLI sensors loaded to maximum density (0.6 pM/cm2) with 100% 2-6 S(LN)2 (Neu5Aca2-6Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAc) or 2-3 S(LN)2 (a, b) or with mixtures of these two synthetic glycans and the asialo glycan LN2 (Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAc) at relative amounts as indicated in the figure (c–f) and illustrated in (g) as an example for panels a, c and e (2-6 Sia, purple diamonds; 2-3 Sia, light green diamonds; galactose, yellow; N-acetylglucosamine, blue).The inserts display the binding rate relative to the maximal binding rate obtained to sensors loaded with 100% 2-6S(LN)2 (a, c, e) or 100% 2-3S(LN)2 (b, d, f). Statistical significance (P) was calculated by two-tailed unpaired t test on biological replicates (a, b n = 6; c–f n = 3). (*P = 0.0493; **P = 0.0085; ***P = 0.0005; ****P < 0.0001). Data are presented as mean values +/−S.D. Panel g was Created with BioRender.com. Source data are provided as a Source Data file.

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