Fig. 2: Low-affinity receptors lower the threshold density for IAV binding by hetero-multivalent interactions to a heterogenous receptor surface. | Nature Communications

Fig. 2: Low-affinity receptors lower the threshold density for IAV binding by hetero-multivalent interactions to a heterogenous receptor surface.

From: Human-type sialic acid receptors contribute to avian influenza A virus binding and entry by hetero-multivalent interactions

Fig. 2: Low-affinity receptors lower the threshold density for IAV binding by hetero-multivalent interactions to a heterogenous receptor surface.

Relative virus binding rates were plotted as a function of relative receptor density for human H3N2 strains VI75 (a), BK79 (b), WU95 (c) and FU02 (d), human H1N1 strain PR8 (e) and avian H5N1 strain HU02 (f). The relative density of the high-affinity receptor is plotted on the x-axis. The black curves display binding to the high-affinity receptor complemented to 100% density with the asialo glycan LN2 (Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAc) whereas the magenta curves display binding to the high-affinity receptor complemented to 100% density with the low-affinity glycans. Relative binding rates are calculated for each point from individual binding curves for each density (see Supplementary Fig. 1 for an example). The absolute binding rates of the different virus strains are plotted in Fig. 3c showing that they differ less than 2-fold. Typical examples from 3 biological replicates are shown for each virus. R-sq values for fitted curves are indicated. Source data are provided as a Source Data file.

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