Fig. 2: Functional validation of DROT1 in drought resistance.
From: Natural variation of DROT1 confers drought adaptation in upland rice
a Drought resistance of drot1-1 compared to IL349. b Drought resistance of DROT1-overexpressing transgenic lines compared to NT (negative transgenic control). The seedlings grown for 4 weeks under normal conditions (top in a, b) were treated by drought stress for 15 days, followed by re-watering for 10 days (bottom in a, b). c Survival rates of IL349 and drot1-1 seedlings after re-watering. Data represent means ± s.d. (n = 3 biological replicates). d Survival rates of NT, OEI and OEY seedlings after re-watering. Data represent means ± s.d. (n = 3 biological replicates). e Growth performance of drot1-1 and IL349 grown in paddy field and severe drought field for 90 days. f Relative plant height of drot1-1 and IL349. Data represent means ± s.d. (n = 15/14 plants). g Relative aboveground biomass of drot1-1 and IL349. Data represent means ± s.d. (n = 10 plants). h Growth performance of NT and OE lines grown in paddy field and drought field for 90 days. i Relative plant height of NT, OEI and OEY. Data represent means ± s.d. (n = 15 plants). The values of relative plant height (f, i) were calculated by plant height in severe drought field divided by those in paddy field. j Relative aboveground biomass of NT, OEI and OEY. Data represent means ± s.d. (n = 7 plants). The values of relative biomass (g, j) were calculated by plant biomass in severe drought field divided by those in paddy field. k Aboveground biomass of drot1-1 and IL349 at mature stage. Data represent means ± s.d. (n = 7/8, 15/14 plants). l Aboveground biomass of NT, OEI and OEY at mature stage. Data represent means ± s.d. (n = 10/11/11, 17/14/16 plants). m Grain yield per plant of drot1-1 and IL349 under paddy field and moderate drought field. Data represent means ± s.d. (n = 29/27, 30/30 plants). n Grain yield per hectare of drot1-1 and IL349 under moderate drought field. Data represent means ± s.d. (n = 3/6 plots). Asterisks indicate statistical significance by two-tailed Student’s t-tests (*P < 0.05, **P < 0.01, ***P < 0.001). Source data are provided as a Source Data file.
