Table 3 Kinetics of SSU head swiveling.

From: Altered tRNA dynamics during translocation on slippery mRNA as determinant of spontaneous ribosome frameshifting

EF-G

kS→N, s−1 (n)a,b

G UUU AAG (non-slippery)

G GGA AAG (slippery)

no EF-G

n.d.

2.5 ± 0.2 (1975)

wt

2.2 ± 0.3 (149)

1.9 ± 0.1 (124)

Q507D

n.d.

<0.1 ± 0.1c (151)

  1. an, number of transitions.
  2. bRates are shown as mean±s.d. from at least three independent experiments. R2 ≥ 0.99 in all cases.
  3. cRate was limited by the photobleaching of the FRET dyes.
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