Fig. 8: Whole-organism CH-RNA-seq mapped neotenic axolotl limb development. | Nature Communications

Fig. 8: Whole-organism CH-RNA-seq mapped neotenic axolotl limb development.

From: Construction of the axolotl cell landscape using combinatorial hybridization sequencing at single-cell resolution

Fig. 8

a Workflow for constructing larval neotenic axolotl single-cell landscape using CH-RNA-seq. b SPRING visualization of 217,781 single cells of larval neotenic axolotl whole-organism and cell-type annotation. c SPRING visualization of limb-development-related cell lineages (left) and limb regeneration blastema cell lineages (right). d MetaNeighbor analysis showing the cell-type correlations of limb-development-related cell lineages and limb regeneration blastema cell lineages (Reg). e Selected downregulated genes from Day 30 to Day 70 post-fertilization. f Selected upregulated genes from Day 30 to Day 70 post-fertilization. Gene ontology (GO) enrichment of downregulated genes (g) and upregulated genes (h) during limb development (p values was calculated by the hypergeometric distribution, statistical test is one-sided, adjustments p values were made after p value is corrected by Benjamin & Hochberg multiple test).

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