Fig. 3: Evaluation of experimental strategy for converting TAG to TAA using single cell RNAseq.
From: Multiplex base editing to convert TAG into TAA codons in the human genome
a Distribution analysis of cells with different number of modified gene targets in populations with three different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. b Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. c For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from method_2 was showed in the plot. d Editing efficiency of each sgRNAs in single cells. e Heatmap of target “C” editing efficiency in the population with different methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
