Fig. 4: Analysis of the genetic changes of highly modified HEK293T clones identified by WGS.
From: Multiplex base editing to convert TAG into TAA codons in the human genome
a Allele editing of all target sites in each clone by Sanger sequencing and EditR. wt—no allele editing; hz (heterozygous) – partial allele editing; hm(homozygous) - all allele editing. b Heatmap of target “C” editing efficiency for converting TAG to TAA. NC, negative control, HEK293T-BE4max stable cell; clone 19 from method_2, clone 21 from method_3; clone 19-1, 19-16, 19-21 from second transfection by method_2. c Number of exonic SNVs (SNVs are located on exons and splicing sites) or other SNVs detected in highly modified clones, as compared to the sequence of the parental HEK293T. The numbers of total SNVs in clone 19, clone 21, clone 19-1, 19-16, 19-21 were 23084, 70356, 35700, 42595 and 31530, respectively. d Number of exonic SNVs detected in essential genes. e Distribution of different types of SNV changes. f Number of detected C·G > T·A SNVs across samples. g Total number of exonic indels or other indels detected in highly modified clones. Highly modified clone means this clone has more edited sites than other clones, and editing efficiency of each edited site is above 3%.
