Fig. 5: On-target and gene expression analysis in highly modified HEK293T clones and lowly modified clones by bulk RNAseq.
From: Multiplex base editing to convert TAG into TAA codons in the human genome
a On-target editing efficiency in two negative control (NC) clones, two lowly modified clones (5, 16), and three highly modified clones (19, 21 and 11). b Transcriptional correlation of wild-type negative control clones and highly modified clones. c Transcriptional correlation of wild-type negative control clones and lowly modified clones. d Volcano plot for differentially expressed genes between lowly modified clones and highly modified clones. e Heatmap for differentially expressed genes between lowly modified clones and highly modified clones. f Expression level of targeted loci in three groups (wild-type negative control clones, lowly and highly modified clones). Highly modified clone means this clone has more edited sites than other clones, and editing efficiency of each edited site is above 3%. The lowly modified clone means the clones have no sites edited after transfection and FACS sorting, wild-type negative control clones were derived from untransfected cells.
