Fig. 3: Structural and biochemical analysis of the ICF mutations on the FF interface.
From: Structure of DNMT3B homo-oligomer reveals vulnerability to impairment by ICF mutations
Close-up view the ICF-associated mutation sites in the DNMT3B-DNMT3B (a) DNMT3B-DNMT3L (b) and DNMT3B-DNMT3L-DNA (c) complexes. The color scheme is the same as that in Fig. 2a. Size-exclusion chromatography analysis of the DNMT3B-DNMT3B (d) and DNMT3B-DNMT3L (e) complexes harboring the ICF mutations on the FF interface. The MTase domain of DNMT3B and the C-terminal domain of DNMT3L were used for the analysis. In vitro DNA methylation assays of the DNMT3B-DNMT3B (f) and DNMT3B-DNMT3L (g) complexes harboring the ICF mutations on the FF interface. Data are mean±s.d. (n = 3 biological replicates). The two-tailed Student t-tests were performed to compare WT vs mutant. Source data are provided as a Source Data file.
