Fig. 3: Recursive Editing increases incorporation of large cargo and therapeutically relevant SNPs.
From: Recursive Editing improves homology-directed repair through retargeting of undesired outcomes
a Carboxy-terminal GFP tagging efficiency of the indicated genes with Recursive Editing in K-562 cells. b Editing outcomes at the indicated target sites without the presence of an HDR donor molecule using Sanger sequencing. c Insertion efficiency of GFP at PPP1R12C (AAVS1) with Recursive Editing in K-562 cells. d Installation efficiency of a chimeric antigen receptor (CAR) at TRAC with Recursive Editing in CD4 + T cells. Left y-axis corresponds to the percentage of cells expressing T cell receptor α/β (TCR) and the right y-axis to the percentage of cells expressing the CAR. g526 was a previously identified high performing gRNA30. Individual editing outcomes at TRAC in CD4 + T cells in the absence of a donor using Sanger sequencing are displayed below. The percentage of alleles targeted by RNP B1 are indicated by the outer curve. e, f Installation of pathogenic ClinVar variants with Recursive Editing in the listed genes in HEK-293T cells. Individual editing outcomes are displayed below for each locus. Each data point represents an individual biological replicate (n = 2–4). Error bars + /− standard deviation (SD). Abbreviations: ins insertion, del deletion, ntc nontargeting control, indels insertions and deletions, WT wildtype. Source data are provided as a Source Data file.
