Fig. 4: Recursive Editing does not increase deleterious genome editing effects.
From: Recursive Editing improves homology-directed repair through retargeting of undesired outcomes
a RPE1 cells (p53 proficient) were electroporated with the indicated reagents and analyzed 24 h later by RT-qPCR for p21 activation. RNP A1, B1, and C1 target UROS. RNP A12 and A13 target PPP1R12C and PLCB4, respectively. The promiscuous RNP targets 14 endogenous target sites18. Each data point represents a biological replicate (n = 3). Error bars + /− standard deviation (SD). b Coverage of PacBio reads containing a deletion at UROS with the indicated combinations of RNPs in HEK-293T cells (n = 1). The inset panel constitutes an overlay with an adjusted y-axis. The dotted line represents the expected DSB site of UROS gA1. c On-target DISCOVER-Seq reads at UROS with the indicated combinations of RNPs in K-562 cells (n = 1). The y-axis denotes the read count. The dotted line represents the expected DSB site of UROS gA1. d Summarized off-targets (OT) in each sample detected by relaxed parameter DISCOVER-Seq for each sample. Dots correspond to positional matches and dashes account for the change in alignment due to 1 and 2 bp insertions. Source data are provided as a Source Data file.
