Fig. 1: Triclosan exposure induces ER stress, stimulates DNL and causes TG accumulation.
From: Lactational delivery of Triclosan promotes non-alcoholic fatty liver disease in newborn mice
WT mice breastfed with TCS or vehicle for 14 or 21 days. a TCS was measured in milk from stomach contents of 14-day old mice (Vehicle n = 4 and TCS n = 6 per group) and serum at 21 days (n = 4 per group). b Expression of hepatic ER stress genes (n = 5 per group) and immunoblot (IB) analysis of total and phosphorylated eIF2α and ATF4 in liver lysate from 21-day old mice (n = 3 mice per group). c Expression of hepatic lipogenic genes at 21 days (n = 6 mice per group) and IB analysis of FASN in livers (n = 3 mice per group). d Measurement of ALT in serum at 21 days old (n = 4 per group) e Measurement of serum palmitate (%), vehicle, n = 4 and TCS, n = 7, serum, n = 6 per group and liver triglycerides (TG), vehicle, n = 4 and TCS, n = 5 at 21 days. f Histology liver sections were stained with H&E and ORO (n = 4 per group), Scale bars=20μm. (a–e) show mean ± S.E., determined by two-tailed Student’s test; *P < 0.05, **P < 0.01, ***P < 0.001.
