Fig. 2: TCS stimulates DNL, ER stress and PPARα in mouse primary hepatocytes and ATF4 induction is PERK dependent.
From: Lactational delivery of Triclosan promotes non-alcoholic fatty liver disease in newborn mice
Mouse primary hepatocytes were isolated from one month old mice and were placed in medium with DMSO or in the presence of 30 µM of TCS. After 72 h of exposure the hepatocytes were collected for Q-RT-PCR analysis. a TCS stimulates genes associated with DNL, n = 4 samples per group. b Downstream PPARα target genes, n = 4 samples per group. c Induction of genes associated with ER stress, n = 4 samples per group. d Hepatocytes were isolated, and cells were transfected with mouse PERK siRNA and control siRNA. Twenty-four hours later, TCS at 30 µM was added in the medium and 48-h later cells were harvested. Q-RT-PCR analysis of Perk after treatment with siRNA (vehicle, n = 3; siRNA Ctrl, n = 4 and siRNA PERK, n = 4). e Representative IB analysis were performed for ATF4. (a–d) show mean ± S.E., determined by two-tailed Student’s test; *P < 0.05, **P < 0.01, ***P < 0.001.
