Fig. 8: Activation of gp130 suppresses liver DNL, PPARα activity and ER stress in liver of TCS breastfed mice.
From: Lactational delivery of Triclosan promotes non-alcoholic fatty liver disease in newborn mice
gp130Act mice received normal milk or TCS through lactation for 21 days. a IB analysis of cytochrome C, caspase 9, cleaved caspase-3 (n = 3 per group). b Expression of claudins and IB of claudin-1 in intestines (vehicle, n = 3 per group and TCS, n = 4 per group). c Expression of YAP/TAZ target genes in the intestines (n = 3 per group). d Expression of hepatic lipogenic genes (gp130Act /veh and WT, n = 4 mice per group and gp130Act /TCS, n = 5). e Expression of hepatic PPARα target genes (gp130Act /veh and WT/TCS, n = 4 mice per group and gp130Act /TCS, n = 5). f Measurement of liver TG (gp130Act /veh and gp130Act /TCS, n = 4 mice per group and WT/TCS, n = 5). g Hepatic genes linked to ER stress (gp130Act /veh and gp130Act /TCS, n = 4 mice per group and WT/TCS, n = 5). h Representative IB analysis of ATF4, FASN and SCD1 (n = 2 per group). i Frozen liver sections were stained with ORO (n = 3 per group). Scale bar, 20μm. (b–g) and (f) show mean ± S.E., determined by two-tailed Student’s test; *P < 0.05, **P < 0.01, ***P < 0.001.
