Fig. 2: Selections against daunomycin yield HFNAPs with binding affinity.
a Selection scheme and parameters are shown. Early rounds utilize reducing conditions to elute binders, to broadly capture HFNAPs. Subsequent rounds rely on target elution with 1 mM daunomycin. Selection 1 (rounds 1–4, 5a-9a) is denoted by the upper path, selection 2 (rounds 4, 5b-8b) by the lower path. b MST characterization of the binding affinity for the top 4 most enriched sequences from selection 1. Sequences were found to bind with Kd = 1–2 µM. Values and error bars reflect the mean and SEM of n = 3 independent replicates. Error bars for some values are too small to extend beyond the data point. c The evolutionary trajectory of the top ten most enriched sequences in selection 1.
