Fig. 1: Schematic illustration of multi-step composition screening of lipid nanoparticles (LNPs) for liver-targeted pDNA delivery. | Nature Communications

Fig. 1: Schematic illustration of multi-step composition screening of lipid nanoparticles (LNPs) for liver-targeted pDNA delivery.

From: Multi-step screening of DNA/lipid nanoparticles and co-delivery with siRNA to enhance and prolong gene expression

Fig. 1

In vitro transfection efficiency was assessed for 1080 LNP formulations with different helper lipids and component ratios. The top-performing formulations for each helper lipid series were then tested in clusters for cytotoxicity and in vivo local transfection efficiency via intrahepatic injection. Clusters that induced minimal cytotoxicity and high transfection were screened via i.v. injection, and LNP formulations within the clusters that demonstrated optimal liver transfection were further evaluated individually.

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