Fig. 3: The inward-facing partially occluded structure with bound cargo.
From: Structures of Atm1 provide insight into [2Fe-2S] cluster export from mitochondria
a The inward-facing partially occluded structure with bound cargo, CtAtm1inw-opn-occl, with the cryo-EM density of bound cargo (gray) and the protein colored as in Fig. 1d. b Close-view of the cargo binding site. The cryo-EM density of the cargo is shown in gray and the modeled glutathione (GSH) molecules as yellow sticks. Asn395 and F396 and residues involved in cargo binding are also shown as sticks. c Comparison of the conformation of transmembrane helix TM6 between the inward-open (CtAtm1inw-opn, gray) and the CtAtm1inw-opn-occl (blue) states, demonstrating a considerable rearrangement of F396. d Identical view as in panel b of ScAtm1 with bound GSH (PDB-ID 4MYH). e Identical view as in panel b of NaAtm1 with bound oxidized GSH (GSSG) (PDB-ID 4MRS). f Identical view as in panel b with the glutathione-bound [2Fe-2S] cluster, [2Fe-2S](GS4), partially fitting in the cargo density.
