Fig. 8: Myosin II filaments in macrophage frustrated phagocytosis.

a Macrophages plated on micropatterned IgG-Alexa 647 disks, with myosin II marked using RLC-EGFP and imaged with TIRF-SIM. Myosin II filaments formed a small ring within the circle of podosomes during macrophage frustrated phagocytosis. Individual myosin II bipolar filaments were visualized as doublets (arrows). The insert, showing a doublet, is a zoom of the dashed box. The scale bar of the insert is 300 nm. b Radial distributions of actin and myosin II (n = 15 sites across 7 cells, data are presented as mean with 95% CI). c Radial distance to the actin and myosin II peaks. Boxes show the median with first and third quartiles (n as in b). Whiskers show the last datum within 1.5*IQR of the box. P values determined using a two-tailed Mann-Whitney U test with Bonferroni correction. d Z-stack of actin 3D-SIM images (lifeact-Halo-549), myosin II (RLC-EGFP), and merged. e 3D view of actin and myosin II filaments shown in d. Source data are provided as a source data file.