Fig. 9: Schematic of podosome constituents drawn to scale.

a Cartoon of actin (magenta) based on iPALM, showing upper radial fibers, lower actin network, and the ventral protrusion. The cross-section shows intensity contours quantified in Fig. 4e. The IgG disk is shown in cyan. Grid unit length = 50 nm. b One-dimensional density plots from TIRF-SIM imaging of species centered on the F-actin peak. Plots correspond to the mean values for each species in Fig. 1e (IgG and FcγRIIA), Fig. 7c (F-Actin, Paxillin, Talin), and Fig. 8b (Myosin II). For IgG and FcγRIIA, white lines correspond to the mean inflection points (Fig. 1f). For paxillin, talin, and myosin, the white lines show the peak and FWHM boundary values. For myosin, the left-hand boundary of the FWHM is beyond the end of the plot but would be at −1.28 μm. PALM/STORM imaging showed cortactin co-localized with actin in both lobes of the podosome (Supplementary Fig. 7).