Fig. 1: Maternal SMCHD1 is required for normal skeletal patterning.
From: Maternal SMCHD1 regulates Hox gene expression and patterning in the mouse embryo
a–c Upper panels: the genetic crosses used to create control (a), maternal null (b) and reciprocal cross heterozygous control (c) embryos for skeletal analysis. The black mouse represents C57BL/6 strain, the brown mouse Castaneus strain. Middle and lower panels: E17.5 skeletons stained with alizarin red (bone) and alcian blue (cartilage), showing a sagittal view of the cervicothoracic region and dorsal view of the thoraco-lumbar-sacral region. The asterisks in b indicate abnormalities compared with the standard axial formulae found in the controls. The skeletons shown are from MMTV-Cre crosses. Scale bar is 5 mm. d The summarised data for all skeletons are provided for MMTV and Zp3-Cre. The asterisks indicate statistical significance in comparing to control (two-tailed chi-square test, degrees of freedom = 1, *** p < 0.001, n = 29 and 28 for the MMTV-Cre and Zp3-Cre control crosses, respectively, n = 33 and 23 for the MMTV-Cre and Zp3-Cre maternal deletion crosses respectively, n = 10 and 21 for Smchd1wt and Smchd1het skeletons respectively from the reciprocal cross using MMTV-Cre). Source data are provided in Supplementary Data 1. e Cartoon depiction of normal skeletal patterning and the Smchd1matΔ phenotype are shown in e for the cervico-thoracic region.
