Fig. 2: Precocious activation of anterior Hox genes in Smchd1 maternal null tailbud samples.
From: Maternal SMCHD1 regulates Hox gene expression and patterning in the mouse embryo
a Graphical depiction of an ~E8 embryo, showing somites in blue and dissection point by a dotted red line, and a summary of the experimental approach. b Tabular summary of replicate and sex data for each embryo at each somite stage dissected. c–d Heatmap of the average reads per million normalised to gene size (RPKM) for the Hox genes at 6 to 11 somites in the control (c) and Smchd1 maternally deleted (d) tailbud samples from the MMTV-Cre model. The colour scheme is given. e Heatmap showing average log2 fold change for expressed Hox genes between the Smchd1 maternal null and control samples. Genes below the expression threshold are denoted with a cross. f As in e but for grouped somite numbers 6–8, 9–11. Source data are provided in Supplementary Data 2. g Whole-mount in situ hybridisation for Hoxc6 in E10.5 embryos of the given genotypes from the MMTV-Cre model. The arrow indicates prevertebra 8 in each embryo, the red bar marks increased anterior expression of Hoxc6 in the Smchd1 maternal null embryo. Scale bar is 1 mm.
