Fig. 3: Precocious activation of anterior Hox genes in differentiating Smchd1 maternal null mESCs upon Wnt activation.
From: Maternal SMCHD1 regulates Hox gene expression and patterning in the mouse embryo
a mESC differentiation to NMP experimental setup, with media components indicated, along with timing of samples taken for RNA-seq. b Heatmap of the average log2 fold change for the expressed Hox genes between the Smchd1 maternal null and control samples. n = 4 from two technical replicates of two mESC lines derived from separate blastocysts for all days except Smchd1matΔ day 2.5 where n = 3 from technical replicates of two independent mESC lines. c Heatmap of the average log2 fold change of Hox gene expression between the day 2.5 and day 2 samples, for each genotype. d The log2 fold change for the Hox1–9 genes between day 2.5 and day 2 of differentiation (n = 27 genes), for the average of the 4 replicates at day 2 and 3 replicates at day 2.5 for each genotype (Student’s t-test, two-tailed, equal variance). Source data are provided in Supplementary Data 3.
