Fig. 1: Effects of Notch and IL-23 signaling for human ILCP differentiation. | Nature Communications

Fig. 1: Effects of Notch and IL-23 signaling for human ILCP differentiation.

From: Notch, RORC and IL-23 signals cooperate to promote multi-lineage human innate lymphoid cell differentiation

Fig. 1

ILC Group 1, 2 and 3 cytokine expression (n = 18; after P/I stimulation) and transcription factors (n = 13) were assessed in bulk ILCP cultures (Supplementary Fig. 2). Expression of Group 1 ILC associated factors (a) IFNγ (b) T-BET (c) and relative quantity of T-BET on OP9-DLL4 vs OP9. Expression of Group 2 ILC associated factors (d) IL-13 (e) GATA-3 (f) and relative quantity of GATA-3 on OP9-DLL4 vs OP9. Expression of Group 3 ILC associated factors (g) IL-17A (h) IL-22 (i) RORγt (j) and relative quantity of RORγt on OP9-DLL4 vs OP9. Data compared using (a–b, d–e, g–i) one-way repeated measures ANOVA using Tukey’s multiple comparison’s test, details in Supplementary Table 1 or (c, f, j) one sample T and Wilcoxon test, details in Supplementary Table 2. ns = not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p < 0.001. Data are presented as (a–b, d–e, g–i) individual unique donors matched across conditions or (c, f, j) mean with SD of ratios of individual donors. Results from from 6 (a, d, g–h) or 4 (b–c, e–f, i–j) independent experiments. Source Data are provided as a Source Data file.

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