Fig. 5: Effects of Notch signaling and IL-23 on clonal ILC differentiation from blood ILCP. | Nature Communications

Fig. 5: Effects of Notch signaling and IL-23 on clonal ILC differentiation from blood ILCP.

From: Notch, RORC and IL-23 signals cooperate to promote multi-lineage human innate lymphoid cell differentiation

Fig. 5: Effects of Notch signaling and IL-23 on clonal ILC differentiation from blood ILCP.The alternative text for this image may have been generated using AI.

Human ILCP cloning on OP9 (n = 20) or OP9-DLL4 (n = 19) stroma with (purple circles) or without (orange circles) IL-23 supplementation. a Frequencies of unipotent (EOMES + ILC1, EOMES-ILC1, ILC2, ILC3, None) and total multipotent clones per individual healthy donor, b Frequencies of types of multipotent clones and c Overall distributions of clones grown in the presence or absence of IL-23 on OP9 stroma. d Frequencies of unipotent and total multipotent clones per individual healthy donor, e Frequencies of types of multipotent clones and f Overall distributions of clones grown in the presence or absence of IL-23 on OP9-DLL4 stroma. Data represented as individual matched values; each point corresponds to an individual donor. Comparisons performed using (a–d) Two-way ANOVA with matching using Šidák’s multiple comparisons test, details in Supplementary Table 5 and e, f chi-square test (observed vs expected; details in Supplementary Table 6). ns = not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p < 0.001. Data represented as individual donors matched across conditions. Source Data are provided as a Source Data file.

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