Fig. 3: Characterization of Vβ8-CAR-T cells after repetitive antigen exposure.
From: T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignancies
a Schematic representation of the CAR-T cell-characterization assay after long-term repetitive antigen stimulation. T cells were transduced with the indicated CAR lentivirus constructs and stimulated with irradiated Vβ8+ Raji cells once every 6 days. On Day 4 after each stimulation, CAR-T cells were studied by flow cytometry and cytotoxicity assays. b Vβ8-CAR-T cells were tested for CAR expression at the indicated time points by flow cytometry. c Vβ8-CAR-T cell growth curves during long-term culture (n = 4 biologically independent samples/group). d Vβ8-CAR-T cells generated as described in Panel A were cocultured with Jurkat cells in triplicate wells at different effector:target (T cell:Jurkat cell) ratios for 24 h (n = 3 biologically independent samples/group). Cytotoxicity was determined by analyzing the remaining IRFP+ cells with flow cytometry. The data shown represent the mean ± SEM from one of three replicate experiments. Statistical significance was determined by two-sided, two-way ANOVA with Sidaks multiple comparisons test.
