Fig. 3: Loss of H3K36me2 has a limited effect on the transcriptomes of FGOs and two-cell embryos.

a Heatmaps showing H3K36me3, H3K36me2, and H3K27me3 enrichment and CG methylation levels in control and K36M FGOs. CG methylation differences between K36M and control FGOs (ΔCGme). 10 kb bins from the whole genome were grouped into five clusters based on H3K36me2 and H3K36me3 enrichment statuses in control FGOs. b Plots showing H3K36me2 enrichment around genic regions in control and K36M FGOs. The analysis was performed for all genes (left, n = 23,081) and those with H3K36me2 loss (right, n = 2407). c Plots showing H3K27me3 enrichment around genic regions in control and K36M FGOs. The analysis was performed for all genes (left, n = 23,081) and those with H3K36me2 loss (right, n = 2407). d MA plots showing changes in gene expression between K36M and control FGOs (left, n = 3) and between K36M oocyte-derived and control late two-cell embryos (right, n = 8). Differentially expressed genes with false discovery rate (FDR) < 0.05, are colored in red. CPM, counts per million. e Boxplots showing expression levels of all genes (n = 23,081) and genes with H3K36me2 loss (n = 2407) in control FGOs (left) and two-cell embryos (right). Genes with H3K36me2 loss included weakly expressed genes, such as olfactory receptor (Olfr) and vomeronasal receptor (Vmnr) genes (Supplementary Data 1). The box shows the median value and 25–75th percentiles, and whiskers show 1.5× interquartile range from the box.