Fig. 5: Simultaneous removal of H3K36me2 and H3K36me3 results in global hypomethylation in FGOs.
a Scatter plots showing CG methylation levels in the control and DM FGOs. Fifty thousand randomly selected 10 kb bins were plotted. b Violin plots showing CG methylation levels in 10 kb bins across the genome in control, Setd2 KO, DM, and Dnmt3a KO7 FGOs. Horizontal bars indicate mean values. c Scatter plot showing CG methylation levels in Dnmt3a KO7 and DM FGOs. Fifty thousand randomly selected 10 kb bins were plotted. d Genome browser snapshots showing CG methylation levels in control, Setd2 KO, DM, and Dnmt3a KO7 FGOs. The upper and lower panels show representative regions from chromosomes 13 and X, respectively. e Western blotting detecting DNMT3A and DNMT3L proteins in DM FGOs. Representative images from three biological replicates. Pan-H3 was used as the loading control. Source data are provided as a Source Data file. f Violin plots showing CG methylation differences between Dnmt3a KO7 and control FGOs in 10 kb bins of DM affected (n = 59,487) and DM unaffected regions (n = 450). Boxplots show median value and 25–75th percentiles, and whiskers show 1.5× interquartile range from the box. g Boxplots showing CG methylation levels in nongrowing oocytes7 in 10 kb bins of DM affected (n = 59,487) and DM unaffected regions (n = 450). The box shows the median value and 25–75th percentiles, and whiskers show 1.5× interquartile range from the box. h A model summarizing H3K36me2/3-dependent CG methylation in mouse oocytes. H3K36me2 and H3K36me3 facilitate CG methylation in MMRs and HMRs, respectively (top). Upon H3K36me2 depletion, CG methylation is lost in MMRs (second), but upon H3K36me3 depletion, CG methylation is lost in H3K36me3-enriched regions and gained in H3K36me2-enriched regions (third). When both H3K36me2 and H3K36me3 are lost, CG methylation is severely lost (bottom). Open and filled circles indicate unmethylated and methylated CG sites, respectively.
