Fig. 1: Triggered transient formation and depletion of a DNAzyme.

a Triggered transient operation of a reaction module consisting of duplex A/A’ (0.2 μM), hairpin H₁ (1 μM) and Mg²⁺-ions, resulting in a Mg²⁺-ion-dependent DNAzyme T₁/A. b Transient concentrations corresponding to: (i)-solid line, the formation and depletion of the Mg²⁺-ion-dependent DNAzyme T₁/A in the presence of trigger T₁ = 0.3 μM, and (i’)-dashed line, computationally-simulated transient using the kinetic model presented in Supplementary Fig. 2. Transient and computational predicted curves (ii)/(ii’), corresponding to the T₁-triggered formation/depletion of DNAzyme T₁/A in the presence of T₁ = 0.2 μM, and (iii)/(iii’) transient and computationally predicted curves, corresponding to the formation/depletion of DNAzyme T₁/A in the presence of T₁ = 0.4 μM. Insert: Time dependent dynamic evolution of T₁/A at short time intervals: (i) in the presence of trigger T₁ = 0.3 μM, (ii) in the presence of trigger T₁ = 0.2 μM, (iii) in the presence of trigger T₁ = 0.4 μM (For the half-life values of the transients, see Supplementary Fig. 6.). c Cyclic operation of the transient DNAzyme cycle (T₁ = 0.4 μM) shown in a. Arrows indicate the addition of the trigger T₁. Source data are provided as a Source Data file.