Fig. 1: Characterization of antigen-encoding mRNAs by flow cytometry or Western blot. | Nature Communications

Fig. 1: Characterization of antigen-encoding mRNAs by flow cytometry or Western blot.

From: Development of a pentavalent broadly protective nucleoside-modified mRNA vaccine against influenza B viruses

Fig. 1

HEK 293 T cells were transfected with HA-, NA- or luciferase control-encoding mRNAs and protein production from each influenza virus antigen-encoding mRNA was assessed via flow cytometry. Positive binding of the antibodies specific for B/Phu HA (a), B/Col HA (b), and B/Col NA (c) relative to luciferase control. HEK 293 T cells were transfected with B/Col NP- or luciferase-encoding mRNAs and protein production from NP-encoding mRNA was assessed via Western blotting. All experiments were performed once. (d) NT non-transfected, L luciferase, GAPDH glyceraldehyde 3-phosphate dehydrogenase. Source data are provided as a source data file.

Back to article page