Fig. 8: A single immunization with the pentavalent IBV mRNA-LNP vaccine protects mice in the nanogram dose range. | Nature Communications

Fig. 8: A single immunization with the pentavalent IBV mRNA-LNP vaccine protects mice in the nanogram dose range.

From: Development of a pentavalent broadly protective nucleoside-modified mRNA vaccine against influenza B viruses

Fig. 8

Mice were I.D. vaccinated once with 5, 0.5, 0.05, 0.005, or 0.0005 µg of each antigen in the pentavalent mRNA-LNP formulation. Four weeks later sera were collected and antibody responses towards B/Colorado/06/2017 (V) influenza virus-infected cells were assessed (p-values from left to right <0.0001, <0.0001) (a). Each symbol represents one animal, and the bar represents the mean. Mice were then I.N. infected with 5mLD50 of B/Malaysia/2506/04 (V) influenza virus and morbidity (b) and survival (c) were monitored for 14 dpi. Data are shown as individual data points and the group average presented as the group mean (n = 5 per group). Significance was assessed using a one-way ANOVA and groups were compared to the luciferase control group at each time point. The dotted line in b indicates the maximum body weight loss (25%) for the experiment. Source data are provided as a source data file.

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