Fig. 2: Cellular localization of genetically edited and tagged ChREBP isoforms in response to glucose.
From: Maladaptive positive feedback production of ChREBPβ underlies glucotoxic β-cell failure
a Design of labeled ChREBP isoforms. mCherry and eGFP were integrated into the genome using the CRISPR/Cas9 method for gene editing in frame with exon 1a and exon 17. b, c Confocal live imaging was performed on Red or Green INS-1 cells after incubation with the indicated concentrations of glucose for 72 h. d The percent nuclear green fluorescence from c was determined. Data are the means ± SEM, n = 3, *P < 0.05; ****p < 0.0001 using one-way ANOVA. e, f Red/Green INS-1 cells were incubated in 2 or 20 mM glucose or the media was changed from 2 to 20 mM glucose as indicated. Confocal live-image microscopy was performed and the percent nuclear green or red fluorescence was determined. The data presented in (e) represent the aggregate of 3 independent experiments. f Representative images from the indicated times and treatments. DAPI nuclear staining is included in the 2 mM image and excluded in the others for clarity. See also related Supplementary Figs. 4–8 and Supplementary Movie 1.
