Fig. 7: Mettl3 is dispensable for homeostasis of adult liver.
a Schematic diagram of tamoxifen-induced liver-specific Mettl3 knockout (Mettl3 icKO) mouse generation. 4 weeks old Mettl3flox/flox/Alb-CreERT2 mice were treated with tamoxifen (Tam) or olive oil (Oil) for 5 consecutive days and then labeled with 0 week. b Western blot for Mettl3 in indicated tissues from Control and Mettl3 icKO mice at 1 week after tamoxifen treatment (3 experiments were repeated independently with similar results). c Western blot for Mettl3 and Hnf4a in Control and Mettl3 icKO mouse liver tissues at different time points after tamoxifen treatment (3 experiments were repeated independently with similar results). d Densitometry analysis of western blot for Mettl3 from Control and Mettl3 icKO mouse liver tissues at different time points after tamoxifen treatment (n = 3 for each group). e RT-qPCR for Mettl3 expression in liver tissues at different time points after tamoxifen treatment (n = 3 for each group over 3 independent experiments). f LC–MS/MS analysis of m6A/A ration in Control and Mettl3 icKO mouse livers 2 weeks after tamoxifen treatment (n = 2 for each group). g Representative gross appearance of livers from Control and Mettl3 icKO mice at 1 week, 2 weeks, 4 weeks, and 6 weeks after tamoxifen treatment (3 experiments were repeated independently with similar results). h Serum levels of AST, ALP, and Cholesterol of Control and Mettl3 icKO mice at different time points after tamoxifen treatment (n = 3 for 6 weeks group; n = 6 for 4 weeks group; n = 10 for other groups). i Representative H&E staining photographs of Control and Mettl3 icKO mouse liver sections at indicated time points (3 experiments were repeated independently with similar results). Scar bar = 100 μm. Data in d–f and h were shown as mean ± SEM with the indicated significance (*P < 0.05, **P < 0.01, ***P < 0.001; two-tailed student’s t-test). Source data are provided as a Source Data file.
