Fig. 1: HLA-C*05 and KIR2DL1 transgenic mouse model. | Nature Communications

Fig. 1: HLA-C*05 and KIR2DL1 transgenic mouse model.

From: Mouse fetal growth restriction through parental and fetal immune gene variation and intercellular communications cascade

Fig. 1

a Schematic of construct used to make HLA-C*05 transgenic mice. Gray shaded region: HLA-C allele, white shaded region: murine H-2Kb allele. b, c Representative cell surface expression of HLA-C*05 on total cells from organs (b) and on gated cell subsets from spleen (c). NK cells are NKp46+CD3-TCR– cells, thymus TCR + cells are CD3+TCR+ and TECs are Epcam+CD45– cells. Numbers denote mean florescence intensity (MFI). d HLA-C cell surface expression plotted as molecules of equivalent fluorochrome (MEF). e Representative cell surface HLA-C*05 expression on spleen CD19+ cells cultured with (solid lines) or without stimulation (dashed lines). Numbers denote MFI. f HLA-C cell surface expression plotted as MEF from CD19+ splenocytes. g Schematic of KIR2DL1 and Ncr1-iCre gene expression construct used to make NK cell-specific KIR2DL1-expressing mice. h Representative cell surface expression of KIR2DL1 on NKp46+CD3-TCR– NK cells in different organs or on different cell subsets from spleen. Numbers denote percentage of KIR+ cells. i Percentage of KIR+ cells in immune cell subsets from the spleen. j, k Cell surface staining of CD27 and CD11b on gated splenic NK cells. Purple: HLA-C*05 mice, gray: WT mice, pink: KIR2DL1-expressing mice, green: HLA-C*05/KIR2DL1 double transgenic mice. Mean ± SEM is shown. n represents biologically independent animals in each group. d n = 4 (WT), 10 (HLA-C*05), for Spleen—CD4+, CD8+, CD19+, CD11c+, and NK cells. n = 3 (WT), 5 (HLA-C*05), for Thymus-TCR+. n = 4 (WT), 5 (HLA-C*05), for Thymus-TECs. f n = 7 (Unstim), 4 (IFNγ stim) and 7 (LPS stim). i n = 9 (WT), 10 (HLA-C*05), 7 (KIR2DL1), 8 (HLA-C*05, KIR2DL1) for NK; n = 4 (WT), 10 (HLA-C*05), 4 (KIR2DL1), 3 (HLA-C*05,KIR2DL1) for CD4, CD8, CD19, and DC. k n = 4 (WT), 10 (HLA-C*05), 4 (KIR2DL1), and 4 (HLA-C*05,KIR2DL1) for all conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Two-tailed Mann–Whitney U-test. Exact p-values are: d HLA-C*05 vs. WT—p = 0.0357 (Thymus TCR + ), p = 0.0159 (Thymus TECs), p = 0.002 (all others). f p = 0.0424 (IFNγ stim vs. Unstim), p = 0.0006 (LPS stim vs. Unstim). i p = 0.0002 (KIR2DL1 vs. WT), p < 0.0001 (HLA-C*05/KIR2DL1 vs. WT). Source Data are provided as a Source Data file.

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