Fig. 6: Mixed bone marrow chimeras show cell-intrinsic exaggeration of a type 2 response in ILCs from Rbm3−/− mice. | Nature Communications

Fig. 6: Mixed bone marrow chimeras show cell-intrinsic exaggeration of a type 2 response in ILCs from Rbm3−/− mice.

From: RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R

Fig. 6: Mixed bone marrow chimeras show cell-intrinsic exaggeration of a type 2 response in ILCs from Rbm3−/− mice.The alt text for this image may have been generated using AI.

a Schematic of bone marrow reconstitution. Mixed bone marrow chimeras were generated using bone marrow from CD45.1 Rbm3+/+ (WT) mice and CD45.2 Rbm3−/− mice and reconstituted for 10–12 weeks, images created with BioRender.com. b Mixed bone marrow chimera mice were challenged 3 times over 7 days with 20 \({{{{{\rm{\mu }}}}}}\)g Alternaria. FACS plots are representative of 2 independent experiments (5 mice each, n = 10) with biological controls. ILCs are gated as Lineage− Thy1.2+ cells. c Percent (p = 0.0178) and total (p = 0.0003) IL5 expressing ILCs from WT vs. Rbm3−/−mice. FACS plots of IL5 percentages. d Percent (p = 0.0012) and total IL13 (p = 0.0029) expressing ILCs from WT vs. Rbm3−/−mice. Representative FACS plots of IL13 percentages. e Percent (p = 0.7764) and total Ki67 (p = 0.0001) expressing ILCs from WT vs Rbm3−/−mice. Representative FACS plots of Ki67 percentages. Paired t-Test, two-tailed. *p < 0.05, **p < 0.005, ***p < 0.0005.

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