Fig. 7: CysLT1R controls eosinophilia and ST2⁺IL-17⁺ ILC2 accumulation in Rbm3^−/− mice.

WT and Rbm3-/- mice were challenged with 25 µg Alternaria three times over 7 days. Lin^-Thy1.2⁺ ILCs were FACS-sorted and bulk RNA-sequenced. Data representative of three mice per group. a Relative transcript levels of select ILC transcripts b PCA plot of WT (blue) and Rbm3^−/− (red) samples. c TPMs of select surface marker, cytokine, and transcription factor transcripts. Unpaired t-test, two-tailed. *p < 0.05, **p < 0.01, ***p < 0.001. d Wild-type, Rbm3^−/−, and Rbm3^−/−Cyslt1r^−/− mice were challenged with Alternaria three times over 14 days. Total type 2 cytokine-producing ILC2s, IL17 producing ST2⁺ILCs, and BAL and lung eosinophils in WT, Rbm3^−/−, and Rbm3^−/−Cysltr1^−/− mice, One^-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Data are presented as mean values+/− SEM.