Fig. 2: GSK3 mediate suppression of fatty acid oxidation triggers proteasome recruitment.

A Mitochondria-associated proteasome activity, proteasome zymography, and K48 ubiquitin levels in mitochondrial fractions in st14 eggs from control-RNAi and GSK3-RNAi ovarioles. (4 replicate experiments on independent biological replicates). B The oxygen consumption rate (OCR) measurements in st14 eggs from control-RNAi and GSK3-RNAi ovarioles (n = 6 biological replicates). C Acyl-carnitine levels from LC/MS analysis of st14 eggs from control-RNAi and GSK3-RNAi ovarioles(n = 8 biological replicates). In the box-and-whisker plots, the box represents the upper and lower quartiles and the line within the box is the mean. The whiskers represent the maximum and minimum values. D Levels of enzymes involved in mitochondrial fatty acid oxidation pathway (FAO) from proteomic analysis of eggs from st1–8 (active growth), st14 (control-RNAi), and st14 (GSK3-RNAi) ovarioles (n = 2 biological replicates). E, F TMRE mitochondrial membrane potential staining of control-RNAi, MTPα-RNAi(E), and ETFα-RNAi (F) ovarioles. Quantification in Figure S2. G OCR measurements in st10 eggs from a control-RNAi, MTPα-RNAi, and ETFα-RNAi ovariole(n = 9 biological replicates). Mitochondria-associated proteasome activity (n = 3 independent experiments on independent biological samples) and K48 ubiquitin levels in mitochondrial fractions from st14 eggs from control-RNAi, MTPα-RNAi (H), and ETFα-RNAi (I) ovarioles. Significance values are calculated by a two -tailed student’s t-test assuming unequal variance. Error bars represent one standard deviation. ^**p < .001, ^*p < .05. Error bars represent 1 X standard deviation.