Fig. 5: Proteasome recruitment to the mitochondria is highly conserved in developmental and starvation-induced forms of quiescence.

A TMRE staining of ovarioles from starved vs fed animals. B Immunostaining wild-type ovarioles to detect Me31B with indicated feeding and treatment strategies. C Summary of percentage of stage 6–8 egg chambers with Me31B aggregates in B (n = 25 egg chambers). P values were calculated by one-way analysis of variance (ANOVA). Error bars represent one standard deviation. D Cytosolic proteasome activity from stage 1–8 egg chambers from fed and starved oocytes (n = 3 completely independent experiments on independent samples 6 total samples). E Fold changes of selected FAO pathway enzymes and VDACs in quiescent NIH 3T3 cells compared with active ones (n = 3 independent replicates). F Total and mitochondria-associated proteasome activity and K48 ubiquitin levels in actively growing and quiescent NIH 3T3 cells(n = 3 independent experiments on multiple samples 7 total samples). G Cytosolic proteasome activity from active and quiescence NIH 3T3 cells (n = 3 completely independent experiments on independent samples (9 samples total)). H Total and Mitochondria-associated proteasome activity and K48 ubiquitin levels in quiescent and germinated N.crassa spores (n = 3 completely independent experiments on independent samples). I Model of how the proteasome participates in either induced or developmental quiescence. Created with Biorender. In the box-and-whisker plots, the box represents the upper and lower quartiles and the line within the box is the mean. The whiskers represent the maximum and minimum values. Significance values are calculated by a two -tailed student’s t-test assuming unequal variance. Error bars represent one standard deviation. ^**p < 0.001, ^*p < 0.05.