Fig. 3: IFN-γ stimulated wild type murine show increased loading of Irga6 onto the PVM of type III parasites. | Nature Communications

Fig. 3: IFN-γ stimulated wild type murine show increased loading of Irga6 onto the PVM of type III parasites.

From: IFN-γ stimulated murine and human neurons mount anti-parasitic defenses against the intracellular parasite Toxoplasma gondii

Fig. 3

Primary neuron cultures were treated as in Fig. 1, followed by infection with listed T. gondii strains. At 12 hpi cultures were fixed and stained with anti-Irga6 and anti-Tuj1 antibodies. The stained cultures were analyzed by confocal microscope. A Representative images of stained cultures from wild type (WT) mice infected with type III parasites. Blue = anti-Tuj1 antibodies, green = anti-Irga6 antibodies, red = mCherry expressing parasites. B Quantification of the percentage of Irga6+ parasitophorous vacuoles (PVs) in the setting of vehicle or IFN-γ pre-stimulation. C Representative images as in A except infected with III + ROP18 parasites. D As in B. E Representative images as in A except using cultures from Irgm1/3 KO mice. A, E Scale bar = 10 µm full image, 5 µm inset. F As in B. G Representative images as in C except using Irgm1/3 KO neurons. H As in B. B, D, F, H Bars- mean ± SEM, N = 100–200 PVs/experiment, 3 independent experiments. ND = not detected. Unpaired t-test with Welch’s correction. Source data are provided as a Source Data file.

Back to article page