Fig. 1: Cellular and molecular characterization of MMc in the offspring’s brain.
From: Pregnancy-induced maternal microchimerism shapes neurodevelopment and behavior in mice
a Experimental set-up of the mating strategies developed to identify MMc in brain based on surface expression of CD45.2 and H-2Db/b in CD45.2/1 and H-2Db/d offspring (left) and based on tdTomato-fluorescent reporter-expression (right). b Number of CD45.2 and H-2Db/b MMc among fetal brain cells on E18.5 (n = 43) and P8 (n = 17), using the gating strategy shown in Supplementary Fig. 1a. c Number of tdTomato-expressing MMc among fetal brain cells on E18.5 (n = 7). d Representative contour plots used to identify tdTomato+ MMc in fetal brain by flow cytometry (left) and tdTomato negative control (right). e UMAP dimensionality reduction plot of scRNA-seq data set from 4305 MMc isolated from fetal brains (n = 21, pooled from 4 litters). The phenotypes of the nine transcriptionally distinct MMc clusters are marked by the color keys. f Dot plots revealing the expression of cluster-specific genes in the nine MMc clusters identified in (e), using the same color keys. The dot size indicates the percentage of expression, the shade represents the average expression within the respective cluster. Data are displayed as mean ± SEM in (b) and (c), each circle corresponds to one pup.
