Fig. 3: Reduction of MMc in the offspring’s brain impairs prefrontal-hippocampal circuits in neonatal mice.
From: Pregnancy-induced maternal microchimerism shapes neurodevelopment and behavior in mice
a Digital photomontage reconstructing the position of a one-shank DiI-labeled electrode in a Nissl-stained 100 µm-thick coronal section containing the prefrontal cortex (top) sub-divided into cingulate cortex area 1 (Cg), prelimbic cortex (PL), and infralimbic cortex (IL) from a P8 mouse. Scale bar, 500 µm. The bottom digital photomontage shows the same but for the CA1 of the hippocampus (HP). Scale bar, 1000 µm. Photomicrographs are representative of three independent experiments. b (top) Extracellular recordings of the local field potential (LFP) in the PL of a P8 MMcpos (left) and MMclow (right) pup after band pass filtering (4–100 Hz) together with the corresponding MUA (500–5000 Hz). (below) Color-wavelet spectra of LFP displayed at identical time scale. (bottom). Same as (top) for HP. c Spectra of absolute power of discontinuous oscillations averaged for all investigated MMcpos (θ n = 11, β n = 10, γ n = 11) and MMclow (θ n = 12, β n = 12, γ n = 11) mice. Dotted lines mark the borders of frequency bands theta (θ) (4–12 Hz), beta (β) (12–30 Hz), and gamma (γ) (30–40 Hz). d Amplitude of oscillations detected in the PL of P8 mice (MMcpos n = 13, MMclow n = 15; p = 0.0022). e, f Same as (c), (d) for the HP (θ MMcpos n = 9, MMclow n = 13; β MMcpos n = 10, MMclow n = 13; γ MMcpos n = 10, MMclow n = 13; Amplitude MMcpos n = 11, MMclow n = 17; p = 0.0109). g Heatmap of coupling between phase and amplitude in MMcpos offspring at P8. h same as (g) for MMclow. i Average phase-amplitude coupling between 20–30 Hz (MMcpos n = 7, MMclow n = 9, p = 0.0007), as shown in (g) and (h). j Log of mutual information between HP and PL (MMcpos n = 11, MMclow n = 15, p = 0.0077). Significance was determined by a two-sided Mann–Whitney U test, *p < 0.05, **p < 0.01. Data in (c) and (e) are displayed as median ± 1.5 IQR. Data in (d), (f), (i), (j) are displayed as mean ± SEM and each circle denotes one pup.
